Measurement

Part:BBa_K4451016:Design

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


pLac-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 871


Design Notes

Team Sheffield 2022 assembled BBa_R0010 into pSB1C3-GFP (BBa_I20270) in place of the constitutive promoter BBa_J23151 via NEB HiFi assembly, to create this part. This part was used as a control to characterise the synthetic lacUV5 promoter BBa_K4451000 (within the transcriptional unit BBa_K4451017) under different IPTG concentrations. Though preliminary experiments found that IPTG-induced cultures expressing BBa_K4451017 gave off noticeably more fluorescence than the control plasmid (BBa_K4451016) at the same optical density, we were unable to collect any quantitative data before the project deadline.

Source

References